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Extraction d’ADN Sang et Cellules

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• Récupération élevée d’ADN de qualité et de différentes tailles
• Protocole court et évolutif
• Extraction peu coûteuse
• Extraction rapide et peu coûteuse
• Produits chimiques non toxiques
• Automatisable sur les systèmes de manipulation de liquides ouverts

Disponible dans une gamme de formats conçus pour répondre aux différents besoins d’échantillonnage et de débit et conçus pour les protocoles manuels et entièrement automatisés.

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Le kit d’extraction d’ADN à base de sang et de cellules magnetiQpermet une méthode rapide et efficace d’isolement d’acides nucléiques à partir d’échantillons de sang entier, ainsi que des suspensions cellulaires. L’extraction de l’ADN se fait par lyse magnétique en une seule étape et capture l’ADN à partir de cellules dans des échantillons de sang, de tissu ou de culture cellulaire.

Réaliser une capture/élution très efficace des acides nucléiques produisant des solutions purifiées avec une contamination minimale des protéines et des sels pour les applications en aval telles que la PCR et le séquençage. Obtenez des concentrations purifiées et élevées d’ADN exempt de débris cellulaires, de sel, de lipides et de protéines en moins de 15 minutes.

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Le kit d’extraction d’ADN sang et cellules magnetiQ réduit le besoin de lyse et de liaisons séparées, de chauffage ou de centrifugation. L’échantillon purifié peut être concentré en volumes aussi bas que 25 μL, contrairement aux kits à base de colonne qui nécessitent souvent des volumes d’élution plus importants. Le kit d’extraction d’ADN sang cellules magnetiQ affiche des performances supérieures par rapport aux principaux kits à base de colonnes disponibles dans le commerce. Une grande quantité d’ADN est extraite de la suspension cellulaire à l’aide du kit d’extraction d’ADN sang et cellules magnetiQ, ce qui entraîne une valeur CT plus faible pour le gène GAPDH.

Le kit d’extraction d’ADN sang et cellules magnetiQ fournit également des préparations d’acide nucléique éluées plus propres et moins contaminées à partir d’échantillons de sang entier ou de suspension de cellules de 100 μL. Le kit produit des élues d’acide nucléique très pures et à haut rendement (rapport entre 1,8 et 2,2) avec une reproductibilité élevée.

Applications

• Séquençage de nouvelle génération (NGS).
• Amplification PCR et qPCR des gènes
• Études de méthylation d’ADN
• Etudes de variation du nombre de copies (VNC)

Caractéristiques

• Se prête aux méthodes à haut débit, y compris l’automatisation
• Collecte magnétique plus rapide et plus simple et étapes de resuspension
• Réduit les risques de colmatage
• Élimine les déchets dangereux de solvants organiques
• Nécessite uniquement l’utilisation d’un rack magnétique et de pipettes standard

[/fusion_tab][fusion_tab title=”Spécifications” icon=””]

Extraction d’ADN Sang et Cellules

Le kit d’Extraction Sang et Cellules magnetiQ contient des tampons optimisés pour la lyse du sang ou des cellules et la liaison, le lavage et l’élution de l’ADN développés avec les billes magnétiques fonctionnalisées de Galenvs et optimisés avec la machine. Cette machine permet de nouvelles approches d’apprentissage pour l’élaboration de protocoles et la formulation de réactifs.

Type d’échantillon	Sang, Cellules, Bactéries
Quantité	100-250 échantillons par cycle
Volume d’élution	25 µL ou plus
ADN récupéré	>98%
Mode de traitement	Automatisé; manuel Compatible avec les plaques à 96 puits.
Rendement	100-250 échantillons par cycle
Technologie de liaison	Billes magnétiques
Capacité de liaison	Extensible
Composants	(i) Tampon de lyse/liaison (ii) Tampon de lavage #1 (iii) Tampon de lavage #2 (iv) Tampon d’élution (v) Protéinase K
Stockage	Température ambiante

[/fusion_tab][fusion_tab title=”Documentation” icon=””]

Extraction d’ADN Sang et Cellules

Guide de démarrage rapide – Extraction d’ADN à base de sang et cellules

Fiche technique – Extraction d’ADN à base de sang et cellules

[/fusion_tab][fusion_tab title=”Protocole vidéo” icon=””]

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