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animation_direction=”left” animation_speed=”0.3″ animation_offset=””]Plasmide Miniprep[/fusion_title][/fusion_builder_column][fusion_builder_column type=”1_2″ layout=”1_2″ align_self=”auto” content_layout=”column” align_content=”flex-start” content_wrap=”wrap” spacing=”” center_content=”no” link=”” target=”_self” min_height=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” class=”” id=”” background_image_id=”” type_medium=”” type_small=”” order_medium=”0″ order_small=”0″ spacing_left_medium=”” spacing_right_medium=”” spacing_left_small=”” spacing_right_small=”” spacing_left=”” spacing_right=”5%” margin_top_medium=”” margin_bottom_medium=”” margin_top_small=”” margin_bottom_small=”” margin_top=”” margin_bottom=”” padding_top_medium=”” padding_right_medium=”” padding_bottom_medium=”” padding_left_medium=”” padding_top_small=”” padding_right_small=”” padding_bottom_small=”” padding_left_small=”” padding_top=”” padding_right=”” 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[/fusion_text][fusion_separator style_type=”none” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” class=”” id=”” flex_grow=”0″ top_margin=”20px” bottom_margin=”” width=”” alignment=”center” border_size=”0″ sep_color=”” icon=”” icon_size=”” icon_color=”” icon_circle=”” icon_circle_color=”” /][fusion_accordion type=”toggles” boxed_mode=”no” border_size=”1″ border_color=”” background_color=”” hover_color=”” divider_line=”” title_font_size=”14px” icon_size=”14″ icon_color=”#fe2560″ icon_boxed_mode=”no” icon_box_color=”” icon_alignment=”” toggle_hover_accent_color=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” class=”” id=””][fusion_toggle title=”Informations supplémentaires” open=”no” class=”MoreInfo” id=””]

• Récupération plasmidique élevée sans RNase
• Protocole court et évolutif pour les vecteurs basse et haute copie
• Extraction peu coûteuse
• Produits chimiques non toxiques
• Automatisable dans des systèmes ouverts

Disponible dans une gamme de formats conçus pour répondre aux différents besoins d’échantillonnage et de débit et conçus pour les protocoles manuels et entièrement automatisés.

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Le kit Plasmide Miniprep de magnetiQ permet une méthode rapide et efficace d’extraction et de purification du plasmide sans besoin de supplément d’RNase. L’extraction de l’ADN plasmidique super huilé est réalisée par une lyse à base de billes magnétiques en une seule étape et la capture de plasmide à partir de bactéries transformées.

Cela permet de réaliser une capture/élution très efficace des plasmides de biologie moléculaire produisant des solutions purifiées avec une contamination minimale d’ARN, de protéines et de sels pour les applications en aval telles que la transfection, la PCR et le séquençage. Le kit Plasmide Miniprep de magnetiQ permet une extraction simple et rapide du plasmide des bactéries cultivées en 15 à 20 minutes à l’aide de nanoparticules magnétiques. Pas besoin de colonnes de spin et de plusieurs étapes de centrifugation Isoler les concentrations très pures d’ADN plasmidique avec un transfert négligeable d’ADNg, de protéines ou d’endotoxines.

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 Plasmide Miniprep

Le kit magnétiQPlasmide Miniprep réduit le besoin d’étapes de centrifugation et de tubes de collecte séparés, contrairement aux kits à colonne standard. magnétiQ Plasmide Miniprep Kit permet une lyse bactérienne facile, une liaison plasmidique et l’élimination de l’endotoxine uniquement à l’aide d’un rack magnétique standard. Le plasmide purifié peut être concentré en volumes aussi bas que 25 μL, par rapport aux kits à base de colonne qui nécessitent souvent des volumes d’élution plus importants. Les performances du kit magnétiQ Plasmide Miniprep démontrent un rendement élevé et une extraction du plasmide pur pour les vecteurs de copie bas et haut. Le traitement des échantillons est terminé en moins de 20 minutes, ce qui permet une grande pureté et une récupération efficace des plasmides de biologie moléculaire.

Applications

• Biologie moléculaire
• Transfection in vitro
• PCR, séquençage, clonage

Caractéristiques

• Se prête aux méthodes à haut débit, y compris l’automatisation
• Collecte magnétique plus rapide et plus simple et étapes de resuspension
• Réduit les risques de colmatage
• Élimine le traitement de RNase
• Élimine les étapes de centrifugation grâce à la lyse bactérienne et à l’élimination des débris cellulaires, des protéines et de l’ADNg dénaturé

[/fusion_tab][fusion_tab title=”Spécifications” icon=””]

 Plasmide Miniprep

Le kit magnétiQ Plasmide Miniprep contient des tampons optimisés pour les applications de biologie moléculaire développés avec les billes magnétiques fonctionnalisées de Galenvs et optimisés avec la machine. Cette machine permet de nouvelles approches d’apprentissage pour l’élaboration de protocoles et la formulation de réactifs.

[/fusion_tab][fusion_tab title=”Documentation” icon=””]

 Plasmide Miniprep

Guide de démarrage rapide – Plasmide Miniprep

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