Custom DNA Oligo Service
Oligonucleotides are routinely used in several molecular biology techniques designed for biomedical research, in vitro diagnostics (IVD), and, more recently, for targeted therapeutics. Regardless of the application, the quality and purity of the oligos are essential to achieve good sensitivity and reproducibility.
At Galenvs Sciences Inc., we are committed to delivering high-quality products to accelerate research and diagnostic accuracy. We offer a spectrum of customized DNA oligonucleotide services to suit your research and development needs. You can now order High-quality custom hydrolysis probes, primers, and modified oligonucleotides for PCR, qPCR, loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), next-generation sequencing (NGS), in-situ hybridization (ISH), CRISPR, and various other applications.
What sets Galenvs apart?
Premium Quality
There is no compromise when it comes to the quality of the oligo synthesis, that’s why Oligos from Galenvs are GMP Grade and in accordance with cGMP guidelines.
High Purity
High purity means low background noise and better reliability. Probes and primers from Galenvs are PAGE or HPLC purified with over 90% full-length product. QC by Mass Spectrometry.
Scale
Be it research or diagnostic development, the reproducibility of your assays matters! Custom oligo synthesis at Galenvs offers a scale of 50 µmol to 100 µmol to suit your experiments.
Primers and Probes to Suit Your Research Requirements
Selecting the right probe combination is crucial for achieving the desired results of your experiments. Galenvs offers high-purity, fit-for-purpose oligonucleotide primer and probe synthesis that deliver robust results. Choose from our wide range of custom modifications that maximize oligonucleotide design and utility possibilities:
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Locked Nucleic Acid (LNA)
LNAs are highly stabilized nucleic acid analogs containing a 2′-O, 4′-C methylene bridge. The improved structural stability of LNAs results in a higher hybridization melting temperature (Tm) and resistance to nucleases. This renders them suitable for optimized aptamer synthesis and precise IVD applications requiring SNP and allelic mutation detection.
Minor Groove Binder (MGB)
3′ MGB-conjugated probes offer high structural stability and specificity with increased Tm. Conjugation of MGBs enables to use short probes for higher specificity, especially for IVD applications.
GMP Grade Primers
Galenvs produces high-quality primers with high purity and good analytical sensitivity for qPCR and NGS workflows for in-vitro diagnostic use.
Molecular Beacons
Molecular beacons are dual-labeled oligo probes with a hairpin-loop structure. Compared to linear probes, their multiplexing ability and improved discrimination of single base-pair mismatch make them suitable for IVD applications, including isothermal LAMP-specific probes.
Fluorophores and Quenchers
Galenvs provides an extensive array of fluorophores (e.g., FAM, HEX, or Cy5) and quenchers (e.g., BHQ1 or BHQ2) to choose from, with options for hydrolysis probes as well as static and FRET quenching mechanisms.
Functional Groups Addition
The addition of functional groups (e.g. amine, thiol, or carboxylate groups) at specific sites within the oligo chain can be used for downstream solid-substrate anchoring on microarray slides, polymeric beads, and magnetic nanoparticles (MNPs), as well as improved conjugation of reporters, enzymes, substrates or other proteins for diagnostic and drug delivery applications.
