RNA sequencing (RNA-seq) is a technique to identify RNA sequences in a biological sample. RNA-seq can be applied to various cell or tissue samples to elucidate physiological and pathological conditions.
Powered by the high-throughput Next Generation Sequencing (NGS) technology, RNA sequencing can provide insights into the dynamic nature of the RNA transcriptome. Apart from studying mRNA expression levels, RNA-Seq data can help discover novel gene transcripts, alternatively spliced isoforms and allele-specific expression. The technique also helps understand a cell’s functional and regulatory complexities by analyzing various RNA species, including pre-mRNA, micro-RNA, small nuclear RNA, and long ncRNA.