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animation_direction=”left” animation_speed=”0.3″ animation_offset=””]Nettoyage PCR[/fusion_title][/fusion_builder_column][fusion_builder_column type=”1_2″ layout=”1_2″ align_self=”auto” content_layout=”column” align_content=”flex-start” content_wrap=”wrap” spacing=”” center_content=”no” link=”” target=”_self” min_height=”” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” class=”” id=”” background_image_id=”” type_medium=”” type_small=”” order_medium=”0″ order_small=”0″ spacing_left_medium=”” spacing_right_medium=”” spacing_left_small=”” spacing_right_small=”” spacing_left=”” spacing_right=”5%” margin_top_medium=”” margin_bottom_medium=”” margin_top_small=”” margin_bottom_small=”” margin_top=”” margin_bottom=”” padding_top_medium=”” padding_right_medium=”” padding_bottom_medium=”” padding_left_medium=”” padding_top_small=”” padding_right_small=”” padding_bottom_small=”” padding_left_small=”” padding_top=”” padding_right=”” 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• Récupération élevée d’ADN de qualité et de différentes tailles
• Protocole court et évolutif
• Extraction rapide et peu coûteuse
• Produits chimiques non toxiques
• Automatisable dans des systèmes ouverts

Disponible dans une gamme de formats conçus pour répondre aux différents besoins d’échantillonnage et de débit et conçu pour les protocoles manuels et entièrement automatisés.

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Le kit de nettoyage PCR magnetiQ permet une méthode rapide et efficace de purification des acides nucléiques à la suite de réactions enzymatiques telles que la PCR ou d’autres processus tels que les digesteurs de restriction ou les réactions de ligature. La purification de l’ADN est obtenue par capture magnétique à base de billes et élution ultérieure dans un tampon propre.

Réaliser une purification rapide des produits d’amplification de PCR en moins de 10 minutes Obtenir un rendement élevé d’ADN exempt de sel et de contamination de protéine. Éliminer le besoin de colonnes de spin et de centrifugation avec capture et purification d’ADN à base magnétique Les échantillons sont traités manuellement en moins de 10 minutes, nécessitant uniquement l’utilisation d’un rack magnétique et de pipettes standard.

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Nettoyage PCR

Le kit de nettoyage PCR magnetiQ diminue les besoins de centrifugation et les tubes de collecte séparés, contrairement aux kits standard à colonne. L’efficacité de capture du Clean-Up PCR de magnetiQ est supérieure à 98 % de l’ADN dans la plage de taille de 100 bps à 10 kbps. > L’échantillon purifié peut être concentré en volumes aussi bas que 10 μL, par rapport aux kits à base de colonne qui nécessitent souvent des volumes d’élution plus importants. La performance du kit de nettoyage PCR magnetiQ démontre une grande efficacité de purification à différentes tailles de fragments. Le traitement des échantillons est terminé en moins de 10 minutes, ce qui donne une grande pureté et une récupération efficace de l’ADN.

Applications

• Séquençage de nouvelle génération (NGS).
• Amplification des gènes post-PCR et qPCR
• Préparation de la bibliothèque d’ADN génomique
• Etudes de variation du nombre de copies (VNC)

Caractéristiques

• Se prête aux méthodes à haut débit, y compris l’automatisation
• Collecte magnétique plus rapide et plus simple et étapes de resuspension
• Réduit les risques de colmatage
• Élimine les déchets dangereux de solvants organiques

[/fusion_tab][fusion_tab title=”Spécifications” icon=””]

Nettoyage PCR

Le kit de nettoyage PCR magnetiQ contient des tampons optimisés pour la liaison, le lavage et l’élution de l’ADN développés avec les billes magnétiques fonctionnalisées de Galenvs et optimisés avec la machine. Cette machine permet de nouvelles approches d’apprentissage pour l’élaboration de protocoles et la formulation de réactifs.

[/fusion_tab][fusion_tab title=”Documentation” icon=””]

Nettoyage PCR

Guide de démarrage rapide – Nettoyage PCR

Fiche technique – Nettoyage PCR

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