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Blood & Cell DNA Extraction

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• High DNA recovery and quality of varying sizes
• Short and scalable protocol
• Cost effective extractions
• Rapid and cost-effective extractions
• Non-toxic chemicals
• Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

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The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, as well as cell suspensions. Extraction of DNA is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.

Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of DNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.

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The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. High quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.

The magnetiQ Blood & Cell DNA Extraction Kit also provides a cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.

Applications

• Next Generation Sequencing (NGS)
• PCR and qPCR gene amplification
• DNA methylation studies
• Copy number variation (CNV) studies

Features

• Amenable to high-throughput methods, including automation
• Faster and simpler magnetic collection and resuspension steps
• Reduces concern for clogging
• Eliminates organic solvent hazardous waste
• Requires only the use of a magnetic rack and standard pipettes

[/fusion_tab][fusion_tab title=”Specifications” icon=””]

  Blood & Cell DNA Extraction

The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.

Sample Type	Blood, Cells, Bacteria
Quantity	100 or 250 assays (Other formats available)
Elution Volume	25 µL or above
DNA recovered	>98%
Processing Mode	Automated; Manual. Compatible with 96-well plates.
Throughput	100-250 samples per run
Binding Technology	Magnetic beads
Binding Capacity	Scalable
Components	(i) Lysis/Binding Buffer (ii) Wash Buffer #1 (iii) Wash Buffer #2 (iv) Elution Buffer (v) Proteinase K
Storage	Room temperature

[/fusion_tab][fusion_tab title=”Documentation” icon=””]

Blood & Cell DNA Extraction

Quick Start Guide – Blood & Cell DNA Extraction

Tech Spec Sheet – Blood & Cell DNA Extraction

[/fusion_tab][fusion_tab title=”Video Protocol” icon=””]

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